Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-β-Tubulin Mouse mAb [88D53]
抗β-Tubulin小鼠单克隆抗体 [88D53]
Cat. No.
A32081
Clone No. 88D53
Synonymsβ-Tubulin, Tubulin beta chain (Tubulin beta-5 chain)
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Antigen-affinity-purified
Concentration
Please check the information on the tube.
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
50 kDa
Observed MW
50 kDa
Clonality
Monoclonal
Clone No.
88D53
抗原与靶点信息 Antigen & Target Information
Gene Name
TUBB
Protein Name
Beta-tubulin
Domain
human TUBA3C/E, aa 400-500
Background
β-Tubulin belongs to tubulin family and controls microtubule dynamics, cell division and molecular motor anchoring. β‑Tubulin forms heterodimers with α‑Tubulin and constitutes microtubule plus end, governing dynamic instability for microtubule growth and shrinkage. Multiple tissue-specific β-tubulin isoforms exist, such as neuron-specific βIII-Tubulin (Tuj1) involved in neuronal differentiation. β-Tubulin is stably expressed across most cell types and used as loading control for WB and IF. Isoform switching or mutations may confer resistance to taxane-class microtubule-targeting drugs. It is widely applied in cytoskeleton, mitosis, neuronal differentiation and anti-tumour drug resistance researches.
Localization
Cytoplasm
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:500-1:2000
IF
1:100-1:500
ELISA
1:1000-1:5000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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