Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Smad3 Mouse mAb [86F19]
抗Smad3小鼠单克隆抗体 [86F19]
Cat. No.
A33115
Clone No. 86F19
SynonymsSmad3, LDS3, mad3, LDS1C, MADH3, JV15-2, hMAD-3, hSMAD3, HSPC193, HsT17436
详细信息 Product Information
Buffer
0.05% preservative in PBS (pH 7.2-7.4)
Purification
Affinity-purified
Concentration
1 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
48 kDa
Clonality
Monoclonal
Clone No.
86F19
Isotype
IgG
抗原与靶点信息 Antigen & Target Information
Gene Name
SMAD3
Protein Name
Mothers against decapentaplegic homolog-3
Domain
Full length
Background
SMAD3 (Smad3) is TGF-β-pathway receptor-activated R-Smad highly homologous to Smad2, transducing TGF-β and Activin signals for cell-cycle arrest, EMT, fibrosis and immune regulation. TGF-β type-I receptor phosphorylates C-terminal SSXS motif of Smad3. Activated Smad3 heterodimerizes with Smad4, translocates to nucleus and binds target-gene promoters to execute TGF-β transcriptional responses. Smad3 shares partial redundancy with Smad2 yet exhibits distinct tissue preference and target-gene selectivity, and is central to organ fibrogenesis. It mediates anti-proliferative effects in early-stage tumors and promotes invasion-metastasis at advanced stages.
Localization
Cytoplasm, Nucleus
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:1000-1:2000
IHC
1:200-1:2000
ICC/IF
1:100-1:500
IF-P
1:200-1:2000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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