Anti-pan Actin Rabbit mAb [10D78]

抗pan Actin兔单克隆抗体 [10D78]

Cat. No. A52001 Clone No. 10D78

Synonymspan Actin, Pan-Actin, β-Actin, γ-Actin

Host species Rabbit
Reactivity Human, Mouse, Rat
Applications WB, IHC, IP, IF, ELISA

详细信息 Product Information

Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
42 kDa
Observed MW
42 kDa
Clonality
Monoclonal
Clone No.
10D78
Isotype
IgG, Kappa

抗原与靶点信息 Antigen & Target Information

Gene Name
ACTB / ACTG1
Protein Name
Pan-actin
Background
Actin (pan) belongs to the actin family and builds the cytoskeleton to support cell motility and intracellular transport. Actin is a highly conserved cytoskeletal protein ubiquitously expressed in all eukaryotic cells, existing both as monomeric G-actin and polymeric filamentous F-actin. It assembles the microfilament network to maintain cell morphology and mechanical tension, and participates in cell migration, adhesion, division, vesicular trafficking and muscle contraction. Distinct isoforms are differentially distributed between muscle and non-muscle cytoskeletons.
Localization
Cytoskeleton
Research Area
细胞生物学
数据库链接
物种 Organism基因 IDSwissProt
Human 70; 58; 59; 60; 71; 72 P62736; P68032; P60709; P63261; P68133; P63267
Mouse 11475; 11464; 11465; 11468; 11459; 11461

实验信息 Product Usage & Documents

推荐稀释比 Recommended Dilution

WB
1:1000-1:5000
IHC
1:200-1:1000
IP
1:50-1:200
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议,实际需按样本类型与显色体系优化。

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