Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-pan AKT (phospho Ser473) Rabbit mAb [46C96]
抗pan AKT (phospho Ser473)兔单克隆抗体 [46C96]
Cat. No.
A52006
Clone No. 46C96
Synonymspan AKT (phospho Ser473), p-Akt-pan(Ser473), p-AKT(S473) Pan-Specific
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
55 kDa
Observed MW
60 kDa
Modification
Phospho
Clonality
Monoclonal
Clone No.
46C96
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
AKT1, AKT2, AKT3
Protein Name
pan AKT (phospho Ser473)
Background
Phospho-AKT (Ser473) belongs to the activated form of pan-Akt serine/threonine kinase family, covering the phosphorylated status of Akt1, Akt2 and Akt3 isoforms. Ser473 is a critical regulatory phosphorylation site located at the C-terminal hydrophobic motif of Akt, whose full phosphorylation is essential for complete Akt kinase activation and maximal downstream signaling transduction. Activated p-Akt (Ser473) acts as a central effector of the PI3K signaling cascade, participating in the regulation of cell survival, anti-apoptosis, glucose metabolism, cell cycle progression and tumorigenesis. Dysregulated phosphorylation of Akt Ser473 is frequently observed in malignant tumors, insulin resistance, inflammatory diseases and tissue fibrosis, serving as a key indicator of aberrant PI3K/Akt pathway activation.
Localization
Cytoplasm, Nucleus
Research Area
癌症
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:1000-1:5000
IHC
1:200-1:500
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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