Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-β-Catenin (phospho Thr41/Ser45) Rabbit mAb [96C82]
抗β-Catenin (phospho Thr41/Ser45)兔单克隆抗体 [96C82]
Cat. No.
A52042
Clone No. 96C82
Synonymsβ-Catenin (phospho Thr41/Ser45), p-β-Catenin(Thr41/Ser45), ARMADILLO, ARM, CATNB, BCL9
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
86 kDa
Observed MW
92 kDa
Modification
Phospho
Clonality
Monoclonal
Clone No.
96C82
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
CTNNB1
Protein Name
β-Catenin (phospho Thr41/Ser45)
Background
β-catenin (Catenin beta-1) belongs to the cadherin-associated protein family and mediates cell-cell adhesion as well as Wnt signal transduction. Thr41/Ser45 are conserved N-terminal phosphorylation sites critical for the degradation cascade of β-catenin. Under Wnt-off status, CK1α initiates phosphorylation at Ser45, followed by sequential phosphorylation of Thr41, Ser33 and Ser37 catalyzed by GSK-3β. Fully phosphorylated β-catenin is recognized by β-TrCP ubiquitin ligase and subjected to ubiquitin-proteasomal degradation to repress downstream transcription. Wnt stimulation inhibits this phosphorylation cascade, leading to β-catenin accumulation and nuclear translocation to activate target genes governing stemness, epithelial-mesenchymal transition and tumor metastasis. Thr41/Ser45 phosphorylation level reflects destruction complex activity and is routinely used to evaluate Wnt pathway status.
Localization
Cytoplasm
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:2000-1:10000
IP
1:50-1:200
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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