Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Cdc25C Rabbit mAb [81B31]
抗Cdc25C兔单克隆抗体 [81B31]
Cat. No.
A52070
Clone No. 81B31
SynonymsCdc25C, MPM2, CDC25A2, mitosis-specific protein phosphatase
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
53 kDa
Observed MW
60 kDa
Clonality
Monoclonal
Clone No.
81B31
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
CDC25C
Protein Name
Cell division cycle 25C
Background
Cdc25C (Cell-division-cycle 25C) belongs to dual-specificity phosphatase family and governs G2/M cell-cycle transition. Predominantly cytoplasmic Cdc25C removes inhibitory phosphate moieties from Cdc2/Cdk1 to activate Cdc2-cyclin B complex and trigger G2-to-M progression. Cdc25C activity is tightly controlled by phosphorylation. Phosphorylation at inhibitory sites sequesters Cdc25C in cytoplasm and arrests cell cycle; dephosphorylation enables nuclear translocation for mitosis promotion. Under DNA-damage stress, Chk1/Chk2 phosphorylates Cdc25C to trigger its degradation and activate G2/M checkpoint. Aberrant Cdc25C overexpression is frequently observed in multiple malignancies. It is an essential target for tumor-proliferation and cell-cycle-checkpoint investigation.
Localization
Nucleus
Research Area
癌症
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:2000-1:10000
IHC
1:1000-1:4000
IP
1:50-1:200
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
相关产品 Related Products
Anti-IL-1β Rabbit mAb [99F42]
WBIPIFELISA
Anti-Polycomb Protein SUZ12 Rabbit mAb [36G88]
WBIHCIPIFELISA
价格与规格
该产品暂未录入规格价格，请联系我们获取报价与现货情况。