Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Collagen IV Rabbit mAb [82H79]
抗Collagen IV兔单克隆抗体 [82H79]
Cat. No.
A52085
Clone No. 82H79
SynonymsCollagen IV, Collagen-IV, Procollagen type IV alpha 1 chain, Goodpasture antigen, alpha-1(IV) collagen
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
160 kDa
Observed MW
200 kDa
Clonality
Monoclonal
Clone No.
82H79
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
COL4A1
Protein Name
Collagen type IV alpha-1 chain
Background
Collagen IV belongs to basement-membrane collagen family and builds basement-membrane scaffold for cell adhesion and barrier regulation. As the principal structural component of basement membranes across tissues, Collagen IV is synthesized by epithelial and endothelial cells, self-assembles into meshwork and associates with laminin and nidogen to form intact basement membrane, separating epithelium from stroma and preserving tissue architecture. Basement-membrane degradation and remodeling participate in angiogenesis and embryonic development. Tumor-cell invasion relies on Collagen IV breakdown to breach basement barrier for metastasis.
Localization
Secreted
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:1000-1:5000
IHC
1:200-1:1000
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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