Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Cyclin B1 (phospho Ser126) Rabbit mAb [43A82]
抗Cyclin B1 (phospho Ser126)兔单克隆抗体 [43A82]
Cat. No.
A52091
Clone No. 43A82
SynonymsCyclin B1 (phospho Ser126), p-Cyclin-B1(Ser126), Cyclin B1, CCNB1, p34-cdc2-related
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
48 kDa
Observed MW
55 kDa
Modification
Phospho
Clonality
Monoclonal
Clone No.
43A82
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
CCNB1
Protein Name
Cyclin B1 (phospho Ser126)
Background
Cyclin B1 (Phospho-Ser126) is cyclin B1, the regulatory subunit of MPF complex, governing G2/M transition and mitotic progression. Cyclin B1 associates with Cdc2/Cdk1 to assemble MPF and drive M-phase entry. Ser126 phosphorylation is a critical modification controlling Cyclin B1 nuclear translocation, protein stability and MPF activity. Appropriate Ser126 phosphorylation promotes Cyclin B1 nuclear import and mitosis onset; subsequent dephosphorylation together with ubiquitination triggers Cyclin B1 degradation for mitotic exit. DNA-damage stress perturbs Ser126 phosphorylation and induces G2/M cell-cycle arrest. Phospho-Ser126 level reports mitotic-pathway activation and is applied in cell-cycle, DNA-damage-response and tumour-proliferation researches.
Localization
Cytoplasm, Nucleus
Research Area
癌症
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:1000-1:5000
IHC
1:500-1:2000
IP
1:50-1:200
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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