Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-ERK1/2 (phospho Thr202/Tyr204) Rabbit mAb [51A81]
抗ERK1/2 (phospho Thr202/Tyr204)兔单克隆抗体 [51A81]
Cat. No.
A52113
Clone No. 51A81
SynonymsERK1/2 (phospho Thr202/Tyr204), p-ERK1/2(Thr202/Tyr204), ERK1/2, p42/p44 MAPK, p-ERK1/2(T202/Y204)
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
44 kDa, 42 kDa
Observed MW
44 kDa, 42 kDa
Modification
Phospho
Clonality
Monoclonal
Clone No.
51A81
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
MAPK3, MAPK1
Protein Name
ERK1/2 (phospho Thr202/Tyr204)
Background
Phospho-ERK1/2 (Thr202/Tyr204) is the hallmark modification for full activation of ERK1/2 kinases. The conserved dual phosphorylation sites Thr202/Tyr204 are specifically modified by upstream MEK kinases. Dual phosphorylation at these residues completely activates ERK1/2 kinase activity, promotes nuclear translocation and downstream signaling cascades, and mediates multiple biological effects including cell proliferation, inflammatory activation, metabolic reprogramming and malignant transformation. The phosphorylation level of Thr202/Tyr204 directly reflects the activation status of the MAPK/ERK pathway, and its aberrant elevation is a typical pathological feature of tumors, chronic inflammation, fibrosis and proliferative diseases.
Localization
Cytoplasm, Nucleus
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:2000-1:10000
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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