Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-GAPDH Rabbit mAb [52D12]
抗GAPDH兔单克隆抗体 [52D12]
Cat. No.
A52128
Clone No. 52D12
SynonymsGAPDH
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
38 kDa
Observed MW
38 kDa
Clonality
Monoclonal
Clone No.
52D12
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
GAPDH
Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
Background
GAPDH is a core glycolytic enzyme responsible for glycolytic catalysis and multifaceted cellular homeostasis. GAPDH catalyzes oxidative phosphorylation of glyceraldehyde-3-phosphate, representing a key step in glycolysis. Apart from carbohydrate metabolism, it also participates in non-canonical biological events including DNA repair, apoptosis, signal transduction and vesicular trafficking. This monoclonal antibody recognizes mammalian GAPDH. It is constitutively and highly stably expressed in most cells and tissues, serving as one of the most popular loading controls for WB, IF and other assays. Note that hypoxia, metabolic reprogramming and cell activation may alter GAPDH abundance, so its suitability as reference should be validated per experimental setup. It is widely used in mammalian cell and tissue studies of metabolism, tumor biology and signaling pathways.
Localization
Cytoplasm
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:10000-1:50000
IHC
1:2000-1:5000
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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