Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Hsp27 Rabbit mAb [77F50]
抗Hsp27兔单克隆抗体 [77F50]
Cat. No.
A52174
Clone No. 77F50
SynonymsHsp27, HSP27, HSPB1, Heat shock protein beta-1
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
23 kDa
Observed MW
27 kDa
Clonality
Monoclonal
Clone No.
77F50
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
HSPB1
Protein Name
Heat-shock protein-27
Background
HSP27 (Heat shock protein 27) belongs to the small heat-shock protein (sHSP) family and resides mainly in cytoplasm, forming oligomeric complexes for chaperone activity. It binds denatured polypeptides and prevents irreversible protein aggregation to shield cells from heat shock, oxidative stress and cytotoxic insults. HSP27 participates in cytoskeleton remodeling and anti-apoptotic regulation to control cell survival and migration. Phosphorylation remodels its oligomer assembly and switches biological activities. Elevated HSP27 is frequently detected in multiple malignancies, correlating with metastasis and chemoresistance; it also contributes to inflammation and neuronal injury.
Localization
Cytoplasm, Nucleus
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:2000-1:10000
IHC
1:200-1:1000
IP
1:50-1:200
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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