Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Iba1 Rabbit mAb [46F91]
抗Iba1兔单克隆抗体 [46F91]
Cat. No.
A52179
Clone No. 46F91
SynonymsIba1, Iba-1, IBA1, GAREF, AIF-1
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
17 kDa
Observed MW
17 kDa
Clonality
Monoclonal
Clone No.
46F91
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
AIF1
Protein Name
Ionized calcium-binding adapter molecule-1
Background
Iba1 (Ionized calcium-binding adapter molecule 1) is an EF-hand calcium-binding protein specifically expressed in microglia and macrophages. It senses calcium signals and regulates cytoskeleton rearrangement to mediate myeloid-cell migration, phagocytosis and immune activation. Iba1 expression increases upon microglial activation triggered by CNS injury or neuroinflammation, whereas resting microglia show low Iba1 signal.
Localization
Cytoplasm
Research Area
神经科学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:1000-1:5000
IHC
1:2000-1:4000
IP
1:50-1:200
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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