Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-LC3B Rabbit mAb [72H51]
抗LC3B兔单克隆抗体 [72H51]
Cat. No.
A52205
Clone No. 72H51
SynonymsLC3-B, LC3B, MAP1LC3B
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
14 kDa, 16 kDa
Observed MW
14 kDa, 16 kDa
Clonality
Monoclonal
Clone No.
72H51
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
MAP1LC3B
Protein Name
Microtubule-associated protein-1 light chain-3 beta
Background
LC3B (Microtubule-associated protein light chain 3B) is the most widely-studied isoform within LC3/GABARAP autophagy family. Soluble LC3B-I resides in cytosol; lipid-modified LC3B-II associates with autophagosome membrane to support autophagosome assembly and serves as autophagosome reporter. Autophagy activation elevates LC3B-II levels, yet autophagic-flux blockage also causes LC3B-II accumulation; flux assays with lysosomal inhibitors are required for correct interpretation.
Localization
Autophagosome Membrane
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:1000-1:5000
IHC
1:200-1:1000
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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