Anti-NF-κB p65 (phospho Ser536) Rabbit mAb [83F08]

Protein A affinity purification

NF-κB p65 (phospho Ser536)

RELA (NF-κB p65, RELA proto-oncogene, NF-κB subunit) is NF-κB transcription-factor family protein governing inflammation, immune response and cell survival. Phosphorylation at Ser536 is critical activating modification catalyzed by IKK and other kinases, triggering dissociation of p65 from cytoplasmic inhibitory complex and enhancing nuclear translocation and transcriptional competence. Under resting conditions p65 is sequestered in cytoplasm. Stimuli including TNF-α and LPS induce Ser536 phosphorylation and nuclear entry to transactivate pro-inflammatory and anti-apoptotic target genes. Constitutive Ser536 phosphorylation occurs in chronic inflammation, autoimmune diseases and malignancies. Ser536 phosphorylation does not dominate DNA binding but modulates co-factor recruitment, enabling discrimination between total and activated p65.

Nucleus