Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Nucleolin Rabbit mAb [42E24]
抗Nucleolin兔单克隆抗体 [42E24]
Cat. No.
A52247
Clone No. 42E24
SynonymsNucleolin
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
77 kDa
Observed MW
110 kDa
Clonality
Monoclonal
Clone No.
42E24
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
NCL
Protein Name
Nucleolin
Background
Nucleolin (Nucleolin) is multifunctional nucleolar phosphoprotein family protein mediating rRNA transcription-processing, ribosome assembly and RNA-stability regulation. Predominantly nucleolar, nucleolin also distributes in nucleoplasm, cytoplasm and plasma membrane. It supports Pol-I-driven rDNA transcription, pre-rRNA maturation and ribosome biogenesis. Additional functions include mRNA stabilization, chromatin remodeling and cell-cycle control. Nucleolin is highly abundant in proliferating and tumor cells; cell-surface nucleolin participates in cell adhesion and tumor angiogenesis. Its phosphorylation profile fluctuates across cell cycle and modulates subcellular localization and molecular activities.
Localization
Nucleus
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:500-1:2000
IHC
1:200-1:1000
IP
1:50-1:200
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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