Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-pan Cadherin Rabbit mAb [86A82]
抗pan Cadherin兔单克隆抗体 [86A82]
Cat. No.
A52263
Clone No. 86A82
Synonymspan Cadherin, Pan-Cadherin
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
100 kDa
Observed MW
130 kDa
Clonality
Monoclonal
Clone No.
86A82
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Protein Name
Pan-cadherin
Background
Pan-Cadherin recognizes conserved intracellular domain across cadherin superfamily and broadly detects total abundance of classical cell-cell adhesion molecules. The immunogen corresponds to highly conserved C-terminal cytoplasmic motif shared by E-cadherin, N-cadherin, P-cadherin and other classical cadherins without isoform discrimination. Intracellular tails of classical cadherins recruit catenins and link actin cytoskeleton to mediate Ca2+-dependent homophilic cell-cell adhesion, supporting tissue morphogenesis, cell polarity and cell migration. Pan-cadherin antibody measures global cadherin pool rather than individual isoform.
Localization
Cell membrane
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:2000-1:10000
IHC
1:1000-1:4000
IP
1:50-1:200
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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