Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Cleaved-PARP1 Rabbit mAb [89B75]
抗Cleaved-PARP1兔单克隆抗体 [89B75]
Cat. No.
A52265
Clone No. 89B75
SynonymsCleaved-PARP1, Cleaved-PARP-1, PARP-1, poly(ADP-ribose) polymerase 1, ADP-ribose polymerase
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
25 kDa
Observed MW
25 kDa
Clonality
Monoclonal
Clone No.
89B75
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
PARP1
Protein Name
Cleaved poly(ADP-ribose) polymerase-1
Background
PARP1 (Poly(ADP-ribose) polymerase 1) belongs to PARP-superfamily DNA-repair enzyme family responsible for DNA-damage sensing and poly-ADP-ribosylation. Cleaved PARP1 is a hallmark apoptotic fragment. Full-length PARP1 senses DNA strand breaks and catalyzes PAR-modification to recruit repair machineries. During apoptosis, activated caspase-3/7 cleaves PARP1 at Asp214 to generate 89 kDa C-terminal fragment and 24 kDa N-terminal fragment, abolishing PARP1-mediated DNA-repair capacity. Cleaved-PARP1 antibody exclusively detects apoptotic cleavage product instead of total full-length PARP1.
Localization
Nucleus
Research Area
癌症
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:500-1:5000
IHC
1:200-1:1000
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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