Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Raf-B (phospho Thr401) Rabbit mAb [32B78]
抗Raf-B (phospho Thr401)兔单克隆抗体 [32B78]
Cat. No.
A52290
Clone No. 32B78
SynonymsRaf-B (phospho Thr401), p-BRAF(Thr401), B-raf, v-raf murine sarcoma viral oncogene homolog B1, BRAF1
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
84 kDa
Observed MW
84 kDa
Modification
Phospho
Clonality
Monoclonal
Clone No.
32B78
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
BRAF
Protein Name
Raf-B (phospho Thr401)
Background
BRAF (B-Raf proto-oncogene, serine/threonine kinase) belongs to RAF serine-threonine kinase family and transmits RAS-downstream signals through MEK-ERK mitogen-activated-protein-kinase cascade. Phosphorylation at Thr401 resides within BRAF regulatory domain and participates in kinase-activation control. Triggered by upstream RAS-GTP, Thr401 phosphorylation promotes conformational rearrangement, relieves auto-inhibition and enables MEK phosphorylation to activate ERK signaling for proliferation and differentiation. Somatic BRAF mutations such as V600E lock kinase in constitutively active state and drive melanoma, thyroid carcinoma and other neoplasms.
Localization
Cytoplasm
Research Area
癌症
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:2000-1:10000
IHC
1:200-1:1000
IP
1:50-1:200
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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