Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-ROCK1 Rabbit mAb [18G16]
抗ROCK1兔单克隆抗体 [18G16]
Cat. No.
A52294
Clone No. 18G16
SynonymsROCK1, Rho-associated coiled-coil-containing protein kinase 1, p160ROCK, ROCK-alpha
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
158 kDa
Observed MW
158 kDa
Clonality
Monoclonal
Clone No.
18G16
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
ROCK1
Protein Name
Rho-associated coiled-coil-containing protein kinase-1
Background
ROCK1 (Rho-associated coiled-coil-containing protein kinase 1) is Rho-downstream serine-threonine kinase serving as major RhoA effector controlling actomyosin contractility, cytoskeleton dynamics, cell migration and polarity. Active GTP-RhoA binds ROCK1 and relieves its auto-inhibition. Activated ROCK1 phosphorylates substrates including MLC and MYPT1 to boost myosin-II activity, promoting stress-fiber and focal-adhesion assembly and cellular contractility. ROCK1 also participates in cytokinesis, vesicle trafficking and EMT. Excessive ROCK1 activity drives tumor invasion and metastasis.
Localization
Cytoplasm
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:1000-1:5000
IHC
1:200-1:1000
IP
1:50-1:200
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
相关产品 Related Products
Anti-IL-1β Rabbit mAb [99F42]
WBIPIFELISA
Anti-Polycomb Protein SUZ12 Rabbit mAb [36G88]
WBIHCIPIFELISA
价格与规格
该产品暂未录入规格价格，请联系我们获取报价与现货情况。