Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Smad2 (phospho Ser250) Rabbit mAb [96D77]
抗Smad2 (phospho Ser250)兔单克隆抗体 [96D77]
Cat. No.
A52305
Clone No. 96D77
SynonymsSmad2 (phospho Ser250), p-SMAD2(Ser250), SMAD2
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
58 kDa
Observed MW
58 kDa
Modification
Phospho
Clonality
Monoclonal
Clone No.
96D77
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
SMAD2
Protein Name
Smad2 (phospho Ser250)
Background
SMAD2 (Smad2) is TGF-β-pathway receptor-activated R-Smad. Ser250 locates within regulatory region of Smad2. Its phosphorylation occurs independent of canonical TGF-β-receptor-driven C-terminal activation motif and is catalyzed by diverse kinases. This modification fine-tunes TGF-β output by modulating Smad2 stability, nucleocytoplasmic shuttling and co-regulator interactions. It is functionally distinct from receptor-mediated activating phosphorylation at Ser465/467. Ser250 phosphorylation mediates pathway crosstalk integrating MAPK and other upstream cues.
Localization
Nucleus
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:1000-1:5000
IHC
1:100-1:500
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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