Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Smad2/3 (phospho Thr8) Rabbit mAb [83C15]
抗Smad2/3 (phospho Thr8)兔单克隆抗体 [83C15]
Cat. No.
A52308
Clone No. 83C15
SynonymsSmad2/3 (phospho Thr8), p-SMAD2/3(Thr8), SMAD2, SMAD3
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
48 kDa
Observed MW
62 kDa
Modification
Phospho
Clonality
Monoclonal
Clone No.
83C15
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
SMAD2, SMAD3
Protein Name
Smad2/3 (phospho Thr8)
Background
Thr8 of Smad2/3 locates at N-terminal MH1 domain and is not part of TGF-β-receptor-driven C-terminal activation motif. It is phosphorylated by kinases including MAPK and CDK. Thr8 phosphorylation modulates Smad2/3 nuclear export, DNA-binding capacity and protein stability, mediating crosstalk between TGF-β cascade and cell-cycle / MAPK pathways. It fine-tunes Smad-dependent transcription with context-dependent stimulatory or repressive outcomes.
Localization
Nucleus
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:2000-1:10000
IP
1:50-1:200
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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