Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Smad3 (phospho Ser423/Ser425) Rabbit mAb [26A55]
抗Smad3 (phospho Ser423/Ser425)兔单克隆抗体 [26A55]
Cat. No.
A52310
Clone No. 26A55
SynonymsSmad3 (phospho Ser423/Ser425), p-SMAD3(Ser423/425), SMAD3
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
48 kDa
Observed MW
52 kDa
Modification
Phospho
Clonality
Monoclonal
Clone No.
26A55
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
SMAD3
Protein Name
Smad3 (phospho Ser423/Ser425)
Background
Smad3 Ser423/Ser425 are C-terminal SSXS dual-phosphorylation sites directly catalyzed by TGF-β type-I receptor and serve as specific activation marker for Smad3 cascade. Upon TGF-β-ligand stimulation, type-I receptor kinase mediates dual-phosphorylation at Ser423/Ser425 of Smad3, inducing conformational switch, Smad4 heterocomplex assembly and nuclear import to drive transcriptional programs underlying anti-proliferation, fibrosis and EMT. This C-terminal modification provides direct readout for TGF-β-signal initiation.
Localization
Nucleus
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:2000-1:10000
IHC
1:200-1:1000
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
相关产品 Related Products
Anti-IL-1β Rabbit mAb [99F42]
WBIPIFELISA
Anti-Polycomb Protein SUZ12 Rabbit mAb [36G88]
WBIHCIPIFELISA
价格与规格
该产品暂未录入规格价格，请联系我们获取报价与现货情况。