Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Vimentin Rabbit mAb [15H44]
抗Vimentin兔单克隆抗体 [15H44]
Cat. No.
A52362
Clone No. 15H44
SynonymsVimentin, Vimentin, VIM, intermediate filament protein VIM
详细信息 Product Information
Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
54 kDa
Observed MW
54 kDa
Clonality
Monoclonal
Clone No.
15H44
Isotype
IgG, Kappa
抗原与靶点信息 Antigen & Target Information
Gene Name
VIM
Protein Name
Vimentin
Background
Vimentin is type-III intermediate-filament cytoskeletal protein predominantly expressed in mesenchymal-derived cells, sustaining cell architecture and mechanical resilience. It represents canonical mesenchymal-cell marker. Vimentin is strongly up-regulated during EMT. Elevated vimentin in tumours correlates with mesenchymal phenotype and enhanced invasive-metastatic potential.
Localization
Cytoskeleton
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:1000-1:5000
IHC
1:1000-1:5000
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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