Anti-α-Smooth Muscle Actin Rabbit mAb [99G22]

抗α-Smooth Muscle Actin兔单克隆抗体 [99G22]

Cat. No. A52369 Clone No. 99G22

Synonymsα-Smooth Muscle Actin, α-SMA, ACTA2, SMA, ASMA, alpha-smooth muscle actin

Host species Rabbit
Reactivity Human, Mouse, Rat
Applications WB, IHC, IP, IF, ELISA

详细信息 Product Information

Buffer
50% Glycerol, 0.05% BSA and 0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
0.2 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
42 kDa
Observed MW
42 kDa
Clonality
Monoclonal
Clone No.
99G22
Isotype
IgG, Kappa

抗原与靶点信息 Antigen & Target Information

Gene Name
ACTA2
Protein Name
Alpha-smooth-muscle actin
Background
α-SMA (α-smooth-muscle actin) is contractile actin isoform and canonical biomarker for myofibroblasts. Quiescent fibroblasts barely express α-SMA. Upon tissue injury and fibrosis, fibroblasts differentiate into myofibroblasts with robust α-SMA expression, mediating cellular contraction and extracellular-matrix secretion.
Localization
Cytoskeleton
Research Area
细胞生物学
数据库链接
物种 Organism基因 IDSwissProt
Human 59 P62736
Mouse 11475 P62737

实验信息 Product Usage & Documents

推荐稀释比 Recommended Dilution

WB
1:1000-1:5000
IHC
1:200-1:1000
IP
1:50-1:200
IF
1:200-1:1000
ELISA
1:5000-1:20000
以上为起始稀释比建议,实际需按样本类型与显色体系优化。

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