Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Bcl-2 (phospho Ser70) Rabbit pAb (A72012)
抗Bcl-2 (phospho Ser70)兔多克隆抗体
Cat. No.
A72012
SynonymsBcl-2 (phospho Ser70), Bcl-2, BCL2A, Bcl-2 (p55)
详细信息 Product Information
Buffer
50% Glycerol, 0.5% BSA and 0.02% preservative in PBS (pH 7.2-7.4)
Purification
Antigen-affinity-purified
Concentration
1 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
26 kDa
Observed MW
26 kDa
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
抗原与靶点信息 Antigen & Target Information
Gene Name
BCL2
Protein Name
Bcl-2 (phospho Ser70)
Domain
human BCL-2, around phosphorylation site of Ser70, aa 41-90
Background
Bcl-2 (B-cell lymphoma-2) is a classic anti-apoptotic member of the Bcl-2 protein family encoded by the BCL2 gene, functioning as a central hub balancing cell survival and apoptosis. Predominantly localized on the outer mitochondrial membrane, endoplasmic reticulum and nuclear membrane, Bcl-2 binds and sequesters pro-apoptotic proteins such as Bax and Bad, inhibits mitochondrial pore formation and the release of apoptotic factors, and blocks the endogenous apoptotic pathway to sustain cell survival. Aberrant overexpression of Bcl-2 significantly enhances anti-apoptotic capacity, driving abnormal cell proliferation, drug resistance and malignant transformation. It serves as a critical biomarker for tumor progression, chemotherapy resistance and poor prognosis, as well as a well-established target for tumor targeted therapy.
Localization
Outer mitochondrial membrane, nuclear membrane, endoplasmic reticulum membrane, cytoplasm.
Research Area
癌症
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:500-1:2000
IHC
1:100-1:300
IP
2-5 μg/mg lysate
IF
1:50-1:200
ELISA
1:10000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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