Anti-Caspase-9 Mouse mAb [96E74]
WBIHCIPIF
Anti-Nucleolin Rabbit pAb (A73127)
抗Nucleolin兔多克隆抗体
Cat. No.
A73127
SynonymsNucleolin, NCL, Nucl, Nucleolin, Protein C23
详细信息 Product Information
Buffer
0.05% preservative in PBS (pH 7.2-7.4)
Purification
Protein A affinity purification
Concentration
1 mg/ml
Storage
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping
Frozen
Form
Liquid
Calculated MW
77 kDa
Clonality
Polyclonal
Isotype
IgG
抗原与靶点信息 Antigen & Target Information
Gene Name
NCL
Protein Name
Nucleolin
Background
Nucleolin (Nucleolin) is multifunctional nucleolar phosphoprotein family protein mediating rRNA transcription-processing, ribosome assembly and RNA-stability regulation. Predominantly nucleolar, nucleolin also distributes in nucleoplasm, cytoplasm and plasma membrane. It supports Pol-I-driven rDNA transcription, pre-rRNA maturation and ribosome biogenesis. Additional functions include mRNA stabilization, chromatin remodeling and cell-cycle control. Nucleolin is highly abundant in proliferating and tumor cells; cell-surface nucleolin participates in cell adhesion and tumor angiogenesis. Its phosphorylation profile fluctuates across cell cycle and modulates subcellular localization and molecular activities.
Localization
Nucleus
Research Area
细胞生物学
实验信息 Product Usage & Documents
推荐稀释比 Recommended Dilution
WB
1:1000-1:2000
IHC
1:200-1:2000
ICC/IF
1:100-1:500
ELISA
1:5000-1:20000
以上为起始稀释比建议，实际需按样本类型与显色体系优化。
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